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john doench  (Addgene inc)


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    Structured Review

    Addgene inc john doench
    John Doench, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/john+doench/Human+CRISPR+Activation+Pooled+Library+(Calabrese+P65-HSF)(Pooled+Library+%231000000111%2C+%2392379%2C+%2392380)/bio_rxiv__64898__2026__03__18__712690-283-17-19
    Average 93 stars, based on 37 article reviews
    john doench - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    CRISPR:

    Article Title: The phosphate exporter XPR1 regulates a gasdermin D–independent mature IL-1β secretion pathway in LPS-stimulated human monocytic cells
    Article Snippet: Data analysis was performed using FlowJo software. .. The human Brunello CRISPR knockout pooled library was a gift from David Root and John Doench (Addgene #73179) . .. The gRNA pooled library in lentiCRISPRv2 was amplified and sequenced according to the accompanying online manual ( https://www.addgene.org/pooled-library/broadgpp-human-knockout-brunello/ ).

    Article Title: ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promote immune evasion in HPV-positive head and neck cancer
    Article Snippet: .. The human CRISPR Brunello lentiviral library was a gift from David Root and John Doench (Addgene #73179) . ..

    Article Title: Enhancing gene therapy vectors manufacturing: CEBPA as a master regulator of rAAV biogenesis.
    Article Snippet: .. Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). .. Lenti dCASVP64_Blast was a gift from Feng Zhang (Addgene plasmid # 61425).

    Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems
    Article Snippet: Jurkat E6-1 (ATCC, TIB-152) were originally obtained from the University of North Carolina Tissue Culture facility and grown in RPMI 1640, containing 10% FBS and 10 μg/ml gentamicin. .. The Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). pXPR_502 was a gift from John Doench & David Root (Addgene plasmid # 96923; http://n2t.net/addgene:96923 ; RRID:Addgene_96923). .. In pXPR_502, the sgRNA tetraloop is modified by two PP7 and two MS2 aptamers. pLX-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50662; http://n2t.net/addgene:50662 ; RRID:Addgene_50662). lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963). pcDNA-dCas9-p300 Core was a gift from Charles Gersbach (Addgene plasmid # 61357; http://n2t.net/addgene:61357 ; RRID:Addgene_61357).

    Article Title: Cep192 insufficiency underlies haploid instability in human cells
    Article Snippet: .. Human Calabrese CRISPR Activation Pooled Library Set A ( ) was a gift from David Root and John Doench (Addgene #92379). .. The library was packaged in Lenti-X293T cells by co-transfecting with pMDLg/pRRE, pRSV-Rev, and pMD2.G (gifts from Didier Trono; Addgene plasmids #12251, #12253, and #12259, respectively) , using X-tremeGENE 9 (6365809001, Roche).

    Article Title: Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators
    Article Snippet: .. Human Dolcetto CRISPR Inhibition Pooled Library (Set A and B) was a gift from David Root and John Doench (Addgene #1000000114) . ..

    Article Title: Thiol Scarcity in Cerebrospinal Fluid Renders Leptomeningeal Acute Lymphoblastic Leukaemia Therapeutically Vulnerable to Ferroptosis
    Article Snippet: .. Human Brunello CRISPR knockout pooled library was a gift from David Root and John Doench (Addgene #73179; http://n2t.net/addgene:73179 ; RRID:Addgene_73179) . ..

    Knock-Out:

    Article Title: The phosphate exporter XPR1 regulates a gasdermin D–independent mature IL-1β secretion pathway in LPS-stimulated human monocytic cells
    Article Snippet: Data analysis was performed using FlowJo software. .. The human Brunello CRISPR knockout pooled library was a gift from David Root and John Doench (Addgene #73179) . .. The gRNA pooled library in lentiCRISPRv2 was amplified and sequenced according to the accompanying online manual ( https://www.addgene.org/pooled-library/broadgpp-human-knockout-brunello/ ).

    Article Title: Thiol Scarcity in Cerebrospinal Fluid Renders Leptomeningeal Acute Lymphoblastic Leukaemia Therapeutically Vulnerable to Ferroptosis
    Article Snippet: .. Human Brunello CRISPR knockout pooled library was a gift from David Root and John Doench (Addgene #73179; http://n2t.net/addgene:73179 ; RRID:Addgene_73179) . ..

    Activation Assay:

    Article Title: Enhancing gene therapy vectors manufacturing: CEBPA as a master regulator of rAAV biogenesis.
    Article Snippet: .. Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). .. Lenti dCASVP64_Blast was a gift from Feng Zhang (Addgene plasmid # 61425).

    Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems
    Article Snippet: Jurkat E6-1 (ATCC, TIB-152) were originally obtained from the University of North Carolina Tissue Culture facility and grown in RPMI 1640, containing 10% FBS and 10 μg/ml gentamicin. .. The Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). pXPR_502 was a gift from John Doench & David Root (Addgene plasmid # 96923; http://n2t.net/addgene:96923 ; RRID:Addgene_96923). .. In pXPR_502, the sgRNA tetraloop is modified by two PP7 and two MS2 aptamers. pLX-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50662; http://n2t.net/addgene:50662 ; RRID:Addgene_50662). lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963). pcDNA-dCas9-p300 Core was a gift from Charles Gersbach (Addgene plasmid # 61357; http://n2t.net/addgene:61357 ; RRID:Addgene_61357).

    Article Title: Cep192 insufficiency underlies haploid instability in human cells
    Article Snippet: .. Human Calabrese CRISPR Activation Pooled Library Set A ( ) was a gift from David Root and John Doench (Addgene #92379). .. The library was packaged in Lenti-X293T cells by co-transfecting with pMDLg/pRRE, pRSV-Rev, and pMD2.G (gifts from Didier Trono; Addgene plasmids #12251, #12253, and #12259, respectively) , using X-tremeGENE 9 (6365809001, Roche).

    Plasmid Preparation:

    Article Title: Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems
    Article Snippet: Jurkat E6-1 (ATCC, TIB-152) were originally obtained from the University of North Carolina Tissue Culture facility and grown in RPMI 1640, containing 10% FBS and 10 μg/ml gentamicin. .. The Human Calabrese CRISPR activation pooled library set A was a gift from David Root and John Doench (Addgene #92379). pXPR_502 was a gift from John Doench & David Root (Addgene plasmid # 96923; http://n2t.net/addgene:96923 ; RRID:Addgene_96923). .. In pXPR_502, the sgRNA tetraloop is modified by two PP7 and two MS2 aptamers. pLX-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50662; http://n2t.net/addgene:50662 ; RRID:Addgene_50662). lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963). pcDNA-dCas9-p300 Core was a gift from Charles Gersbach (Addgene plasmid # 61357; http://n2t.net/addgene:61357 ; RRID:Addgene_61357).

    Generated:

    Article Title: Surfaceome CRISPR activation screening uncovers ligands regulating tumor sensitivity to NK cell killing
    Article Snippet: LentiCRISPRv2 (Addgene #52961), lentiCas9-Blast (Addgene #52962), and dCas9VP64_GFP (Addgene #61422) were gifts from Feng Zhang. .. AAVS1 SA-2A-puro- pA (Addgene #22075) was a gift from Rudolf Jaenisch. pXPR_502 (Addgene #96923) was a gift from John Doench and David Root. pSLCAR-CD19-BBz (Addgene #135992) was a gift from Scott McComb. pD649-HAsp-SIGLEC1(Trunc1)-COMP5AP-AviTag- 9xHis (Addgene #157610) was a gift from Chris Garcia. espCas9(1.1)-2A-GFP Gln tRNA plasmids containing gRNAs targeting AAVS1 were gifts from David Schatz. pLV-EF1a-dCas9VP64_2A_EGFP_2A_BSD, pLV-EFS-PCP_p65_HSF1_2A_mcherry_HygR, pLV-U6_sgRNA_pp7-EFS-Puro_2A_BFP, pLV- 2Apuro_2A_BFP, AAVS1-BSD-EF1a_Fluc2_2A_GFP, and pLV-EF1a- Fluc2_2A_GFP_2A_BSD, pLV-puro_2A_BFP, and all cDNA containing plasmids were generated by Gibson Cloning using the NEB Builder HiFi DNA Mastermix (New England Biolabs E2621L). pLV-EF1a-dCas9VP64_2A_EGFP_2A_BSD was generated by inserting a 2A-BSD fragment into dCas9VP64_GFP cut with BsrgI and EcoRI. pLV- EFS-PCP_p65_HSF1_2A_mcherry_HygR was generated by inserting EFS, PP7_p65_HSF1 (from pXPR_502), 2A_mcherry, and 2A_HygR fragments into EF1a- EGFP-2A-ZeoR cut with PacI/EcoRI. pLV-U6_sgRNA_pp7-EFS-Puro_2A_BFP was generated by inserting EFS, Puro, and 2A_mtagBFP fragments into pXPR_502 cut with XhoI/MluI. pLV-2A_puro_2A_BFP was generated by inserting 2A_puro and 2A_BSD fragments into EF1a-EGFP-2A-ZeoR cut with MluI/BamHI. ..

    Cloning:

    Article Title: Surfaceome CRISPR activation screening uncovers ligands regulating tumor sensitivity to NK cell killing
    Article Snippet: LentiCRISPRv2 (Addgene #52961), lentiCas9-Blast (Addgene #52962), and dCas9VP64_GFP (Addgene #61422) were gifts from Feng Zhang. .. AAVS1 SA-2A-puro- pA (Addgene #22075) was a gift from Rudolf Jaenisch. pXPR_502 (Addgene #96923) was a gift from John Doench and David Root. pSLCAR-CD19-BBz (Addgene #135992) was a gift from Scott McComb. pD649-HAsp-SIGLEC1(Trunc1)-COMP5AP-AviTag- 9xHis (Addgene #157610) was a gift from Chris Garcia. espCas9(1.1)-2A-GFP Gln tRNA plasmids containing gRNAs targeting AAVS1 were gifts from David Schatz. pLV-EF1a-dCas9VP64_2A_EGFP_2A_BSD, pLV-EFS-PCP_p65_HSF1_2A_mcherry_HygR, pLV-U6_sgRNA_pp7-EFS-Puro_2A_BFP, pLV- 2Apuro_2A_BFP, AAVS1-BSD-EF1a_Fluc2_2A_GFP, and pLV-EF1a- Fluc2_2A_GFP_2A_BSD, pLV-puro_2A_BFP, and all cDNA containing plasmids were generated by Gibson Cloning using the NEB Builder HiFi DNA Mastermix (New England Biolabs E2621L). pLV-EF1a-dCas9VP64_2A_EGFP_2A_BSD was generated by inserting a 2A-BSD fragment into dCas9VP64_GFP cut with BsrgI and EcoRI. pLV- EFS-PCP_p65_HSF1_2A_mcherry_HygR was generated by inserting EFS, PP7_p65_HSF1 (from pXPR_502), 2A_mcherry, and 2A_HygR fragments into EF1a- EGFP-2A-ZeoR cut with PacI/EcoRI. pLV-U6_sgRNA_pp7-EFS-Puro_2A_BFP was generated by inserting EFS, Puro, and 2A_mtagBFP fragments into pXPR_502 cut with XhoI/MluI. pLV-2A_puro_2A_BFP was generated by inserting 2A_puro and 2A_BSD fragments into EF1a-EGFP-2A-ZeoR cut with MluI/BamHI. ..

    Inhibition:

    Article Title: Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators
    Article Snippet: .. Human Dolcetto CRISPR Inhibition Pooled Library (Set A and B) was a gift from David Root and John Doench (Addgene #1000000114) . ..



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